In vitro preclinical services for
kidney & beyond
NEPHRIX Biosolutions delivers a comprehensive portfolio of mechanistically validated in vitro cellular models, enabling early-stage drug screening, efficacy assessment, and nephrotoxicity profiling – all within a month-long turnaround.
Validate new leads and secure your next go-to in vivo step
Based on our in vitro assays platform, we assess new molecules from your libraries by testing their nephrotoxicity and efficacy -on selected parameters. Exploration of molecular mechanisms validation are guided by Go/No-Go decisions. Characteristics of our innovative solutions :
- Shows >80% of physiological features
- High level of drug effects prediction
- Modulable micro-environment
- Reduction of animal tests failures and costs
At NEPHRIX Biosolutions we provide a comprehensive portfolio of cellular models tailored to diverse research needs:
Fibrosis Assay
We optimized a panel of in vitro fibrosis assays using human primary fibroblasts (Renal, Liver, Lung, Cardiac and Skin) enabling the evaluation of pro-fibrotic related markers : i.e. fibronectin, α-SMA expression, or extra-cellular matrix secretion.
α-SMA – Fibronectin – Collagen- I/III – TGF-β1 – ECM remodelling
Explore the fibrosis assay
DuoXProx™ – Kidney Fibrosis Assay
Our kidney-specific co-culture model combining proximal tubular cells and renal fibroblasts in a single optimised system. Reproduces the cellular crosstalk driving pathological remodelling with unmatched translational relevance..
α-SMA – Fibronectin – Collagen- I/III – TGF-β1 – ECM remodelling
Explore the kidney fibrosis assay
Inflammation Assay
Using human whole blood or isolated immune cells (PBMCs, macrophages), this assay evaluates compound effects on inflammatory signalling. Suited for NF-κB modulators and cytokine inhibitors, with donor-matched comparators ensuring reproducibility across batches.
IL-1β / IL-6 / TNF-α – NF-κB activation – COX-2 – Cytokine multiplex
Explore the inflammation assay
Senescence Assay
Our assay evaluate the different parameters of aging-associated cell dysfunction (SA-beta-Galactosidase assay, SASP, respiration…) and evaluate anti-aging or therapeutic interventions..
SA-β-Galactosidase – SASP panel – p21 / p16 – Mitochondrial respiration
Explore the senescense assay
Nephrotoxicity Screening
Frequently Asked Questions – In vitro preclinical services
We routinely test small molecules, biologics, peptides, and natural extracts for anti-fibrotic, anti-inflammatory, senolytic, senomorphic, or nephroprotective activity, as well as early nephrotoxicity safety screening. Our team advises on solubility, vehicle controls, and concentration range based on your compound’s physicochemical profile.
We work exclusively with human primary cells from defined tissue sources: renal, hepatic, pulmonary, cardiac, and dermal fibroblasts; proximal tubular epithelial cells; and human whole blood or isolated immune cells (PBMCs, macrophages). All primary cells are sourced from validated commercial suppliers and characterised before use. Immortalised cell lines are available on request.
Yes. We regularly design multi-assay packages combining efficacy (fibrosis), mechanism (inflammation), and safety (nephrotoxicity) assessments in parallel. This maximises data per compound, supports comprehensive Go/No-Go decisions, and reduces overall cost and timeline compared to sequential studies.
Why choose NEPHRIX Biosolutions?
Scientific Expertise
Clinically Relevant Models
Flexible Study Design
Multi-parameters Readouts
Why choose NEPHRIX Biosolutions?
Scientific Expertise
Clinically Relevant Models
Flexible Study Design
Multi-parameters Readouts