In Vitro Fibrosis Assays
Customized fibrosis assays for early-stage drug discovery
Fibrosis is a major contributor to the progression of many chronic diseases, underscoring the need for reliable models to understand its mechanisms and evaluate emerging therapies. Our fibrosis assays offer robust in vitro platforms supporting both early-stage screening and drug discovery research. To address this need, we provide two approaches that together form a strong foundation for antifibrotic research and integrated with our inflammation assays enabling a comprehensive, translational approach to therapeutic development:
- a single-culture system based on human primary cells from multiple organs : renal, liver, lung, cardiac and skin
- a kidney-focused co-culture model that captures essential cell–cell interactions.
You have a specific project, contact us and we will be happy to propose you a study plan fitting your specific needs.
Human primary fibroblasts assay
We optimized a panel of in vitro fibrosis assays using human primary fibroblasts (Renal, Liver, Lung, Cardiac and Skin) enabling the evaluation of pro-fibrotic related markers (i.e. fibronectin, α-SMA expression, or extra-cellular matrix secretion) in defined stress conditions :
- Primary Human cells
- Automated images acquisiton and quantification
- Evaluation of fibronectin and intra-cellular α-SMA fibers via a specific fiber-search algorithm
- Dosage of secreted proteins matrix and cytokines
DuoXProx™, the kidney-specific co-culture model
Our DuoXProx™, the kidney-specific co-culture model, combines proximal tubular cells and renal fibroblasts in optimized medium within a single system, enabling the evaluation of fibrosis-related parameters while reproducing the cellular crosstalk driving pathological remodelling.
- Reconstitution of original cellular environment
- Customizable stressors: Hormonal, Diabetic, Nephrotoxic or Hypoxic
- Customizable with multi-parameters analysis
- AI-based image analysis
Additional fibrosis services
- Fibroblast migration – scratch assay; wound closure
- Epithelial migration – scratch assay; wound closure
- In vivo fibrosis models
Related in vitro assays