In vitro Services : Fibrosis Assay

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Fibrosis Assay

Tissue fibrosis, a pathological process characterized by the excessive accumulation of scar tissue in the organs.

In the kidney, this phenomenon plays a critical role in various chronic kidney diseases development, including diabetic nephropathy, interstitial nephritis, and glomerulonephritis. When renal injuries occur in response to infections, inflammation, or other stressors, damaged kidney tissue is gradually replaced by collagen and other proteins, resulting in fibrosis. This process alters the structure and function of the kidneys, potentially leading to a loss of renal function, chronic kidney disease, and even the need for dialysis or kidney transplantation. Understanding renal fibrosis is crucial for the development of new therapeutic approaches aimed at slowing or preventing its progression in the context of kidney diseases.

NEPHRIX Biosolutions has optimized a panel of in vitro fibrosis assays using human primary fibroblasts (renal, lung, cardiac and skin). Our fibrosis assays enable the study of fibrosis related parameters (i.e. fibronectin or alpha-SMA expression,  or extra-cellular matrix secretion) in defined pro-fibrotic conditions

Fibrosis assay

Human primary fibroblasts or epithelial cells from different donors

Available cells: Renal, Lung, Cardiac and Skin

Automated images acquisiton and quantification of 96-wells plates (7 images par well)

Automated quantification of Fibronectin and intra-cellular α-SMA fibers via a specific fiber-search algorithm.

Dosage of secreted proteins matrix and cytokines by ELISA 

Study design

Cells amplification
Cells seeding in 96-well plates
TGF-β induction +/- test items
Media collection and cells fixation
IF staining and dosages

Assay readouts

Kidney Preclinical research