Tissue fibrosis is a pathological process characterized by the excessive accumulation of scar tissue in the organs.
In the kidney, this phenomenon plays a critical role in various chronic kidney diseases development, including diabetic nephropathy, interstitial nephritis, and glomerulonephritis. When renal injuries occur in response to infections, inflammation, or other stressors, damaged kidney tissue is gradually replaced by collagen and other proteins, resulting in fibrosis. This process alters the structure and function of the kidneys, potentially leading to a loss of renal function, chronic kidney disease, and even the need for dialysis or kidney transplantation. Understanding renal fibrosis is crucial for the development of new therapeutic approaches aimed at slowing or preventing its progression in the context of kidney diseases.
Human primary fibroblasts or epithelial cells from different donors
Available cells: Renal, Lung, Cardiac and Skin
Automated images acquisiton and quantification of 96-wells plates (7 images par well)
Automated quantification of Fibronectin and intra-cellular α-SMA fibers via a specific fiber-search algorithm.
Dosage of secreted proteins matrix and cytokines by ELISA