In vitro Services : Inflammation Assay

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Inflammation Assay

Discover our cutting-edge in vitro inflammation assay, developped to provide accurate and reliable results.

Tissue inflammation represents a pivotal pathological condition observed across a broad spectrum of both acute and chronic diseases. 

Our  inflammation  assays  offer a comprehensive platform for studying inflammatory responses in a controlled environment. From drug discovery to understanding disease mechanisms, our dosing technology allows to delve deeper into inflammation pathways, paving the way for breakthroughs in therapeutic development.

Our inflammation assay facilitates the analysis of conventional pro-inflammatory cytokines (LUMINEX or  Ella Automated Immunoassay System) but also delves deeper into the mechanisms underlying cell inflammation through multi-parameter analysis of treated cells. (Gene expression analysis, Immunofluorescence). 

  • Inflammatory panels (IFN-γ, TNF-α, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, CRP and more).
  • Cytokine and Chemokine panels (MCP-1, MCP-4, Eotaxin, IP-10, GM-CSF, IL-15, IL-16, IL-17A, TSLP, TNF- β and more)
inflammation assay

The assay can be customized to suit specific experimental requirements, allowing for the assessment of various cell types, treatments, and conditions.

Highly sensitive ELISA or Multiplex dosage of secreted cytokines by ELLA-multiplex

With multiplex capabilities, our assay enables simultaneous quantification of multiple cytokines, providing a comprehensive profile of the inflammatory response.

Exemple of Study design

Cells amplification
Cells seeding in 24-well plates
High glucose induction +/- test items
Media collection and cells fixation
IF staining and dosages

Exemple of Assay readouts

Primary RPTEC cells response to 5 days in high glucose medium:

Cro| Inflammation assay