UUO Model of Kidney Fibrosis
The gold standard in Renal Fibrosis research – the Unilateral Ureteral Obstruction Model
Unlock the anti-fibrotic potential of your compounds with our Unilateral Ureteral Obstruction (UUO) model. Widely used for assessing renal fibrosis, UUO reproduces key pathological hallmarks of Chronic Kidney Disease, including interstitial fibrosis, tubular atrophy, and inflammatory cell infiltration.
Why Choose Our UUO Model?
- Proven Efficacy: Our UUO model consistently delivers reliable results, allowing to validate a first proof-of-concept in kidney fibrosis.
- Optimized Test Duration: With a concise test duration of just 1 or 2 weeks, our UUO model streamlines in vivo screening tests for anti-fibrotic drugs, saving time and resources without compromising accuracy.
- Comprehensive Analysis: From histopathology to biochemistry and gene expression studies, our model facilitates multi-faceted investigations, maximizing data yield from each experimental subject.
Through precise ligation of one side of the ureter, we induce targeted disease pathology while minimizing procedural variability. Subsequently, the diseased kidney is divided into three sections, enabling thorough analysis across different modalities. From histopathological assessments to biochemical assays and gene expression profiling, our UUO model offers an efficient tool to explore renal fibrosis progression.
Contact us to learn more about how we can support your scientific endeavors with our mice models and rats models of UUO.
Downoad the poster of our UUO Kidney model
C57Bl6/J mice or aternatively on Spargue Dawley rats
Test duration up to 2 weeks, facilitating rapid in vivo screening tests for anti-fibrotic drugs
Multiple parametric analyses, including histopathology, biochemistry, and gene expression studies
Study design
Assay readouts
| Category | Readout / Marker | Methods |
|---|---|---|
| Histopathology | Interstitial fibrosis quantification | Sirius Red staining |
| Collagen deposition | Masson’s Trichrome staining | |
| Tubular atrophy / injury | H&E staining | |
| Fibrosis markers | α-SMA (myofibroblast activation) | Immunohistochemistry / Western blot / qPCR |
| TGF-β1 expression | Gene expression (qPCR) / Dosage (ELISA) | |
| Fibronectin | Immunohistochemistry / Gene expression (qPCR) | |
| Inflammatory | Inflammatory cell infiltration | Immunohistochemistry (F4/80, NFkB) |
Frequently Asked Questions – UUO Model
The Unilateral Ureteral Obstruction (UUO) model is a well-established preclinical tool used to study renal fibrosis and chronic kidney disease (CKD). It reliably reproduces key pathological features including tubulointerstitial fibrosis, tubular atrophy, and inflammatory cell infiltration, making it a gold-standard model for evaluating antifibrotic drug candidates.
NEPHRIX Biosolutions performs the UUO model in C57Bl/6J mice and Sprague-Dawley rats, allowing flexibility depending on your study design and compound requirements.
The UUO model has a test duration of 1 to 2 weeks, making it one of the fastest in vivo fibrosis models available for early-stage compound screening.
Our UUO model provides a comprehensive set of readouts including histopathology (Sirius Red, Masson’s Trichrome), fibrosis markers (α-SMA, collagen deposition), inflammatory markers, and gene expression profiling (TGF-β1, fibronectin). Biochemical analyses are also available upon request.
Yes. The UUO model is widely used for first proof-of-concept (PoC) evaluation of antifibrotic compounds. Its rapid progression and well-characterized endpoints make it ideal for early-stage drug development and Go/No-Go decisions.