Fibrosis Assay
Tissue fibrosis, a pathological process characterized by the excessive accumulation of scar tissue in the organs.
In the kidney, tissue fibrosis plays a critical role in various chronic kidney diseases development, including diabetic nephropathy, interstitial nephritis, and glomerulonephritis. When renal injuries occur, damaged kidney tissue is gradually replaced by an excessive extra-cellular matrix deposition, resulting in fibrosis. This process alters the structure and function of the kidneys leading to a loss function, and even the need for dialysis or kidney transplantation. Understanding renal fibrosis is crucial for the development of new therapeutic approaches aimed at slowing or preventing its progression in the context of kidney diseases.
NEPHRIX Biosolutions has optimized a panel of in vitro fibrosis assays using human primary fibroblasts (renal, lung, cardiac and skin) enabling the study of fibrosis related parameters (i.e. fibronectin or alpha-SMA expression, or extra-cellular matrix secretion) in defined pro-fibrotic conditions.
Contact us to learn more about how we can support your scientific endeavors with our in vitro fibrosis assay.
Human primary fibroblasts or epithelial cells from different donors
Available cells: Renal, Lung, Cardiac and Skin
Automated images acquisiton and quantification of 96-wells plates (7 images par well)
Automated quantification of Fibronectin and intra-cellular α-SMA fibers via a specific fiber-search algorithm.
Dosage of secreted proteins matrix and cytokines by ELISA
Study design
Assay readouts
