Fibrosis Assay
Tissue fibrosis, a pathological process characterized by the excessive accumulation of scar tissue in the organs
When organ injuries occur, damaged tissue is gradually replaced by an excessive extra-cellular matrix deposition, resulting in fibrosis. This process alters the structure and function of the targeted organ leading to a loss function, and even the need for transplantation. Understanding tissue fibrosis is crucial for the development of new therapeutic approaches aimed at slowing or preventing its progression in the context of kidney diseases or related organs.
NEPHRIX Biosolutions has optimized a panel of in vitro fibrosis assays using human primary fibroblasts (Renal, Lung, Cardiac and Skin) enabling the study of fibrosis related parameters (i.e. fibronectin and α-SMA expression, or extra-cellular matrix secretions) in defined pro-fibrotic conditions.
Contact us to learn more about how we can support your scientific endeavors with our in vitro fibrosis assay.
Downoad the poster of our Fibrosis assay
Human primary fibroblasts or epithelial cells from different donors
Available cells: Renal, Lung, Cardiac and Skin
Automated images acquisiton and quantification of 96-wells plates (7 images par well)
Automated quantification of Fibronectin and intra-cellular α-SMA fibers via a specific fiber-search algorithm.
Dosage of secreted proteins matrix and cytokines by ELISA
Study design
Assay readouts
