In vitro Services : Inflammation Assay

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Inflammation Assay

Discover our cutting-edge in vitro inflammation assay, developped to provide accurate and reliable results.
 

Tissue inflammation represents a pivotal pathological condition observed across a broad spectrum of both acute and chronic diseases. 

Our  inflammation  assays offer a comprehensive platform for studying inflammatory responses in a controlled environment. From drug discovery to understanding disease mechanisms, our dosing technology allows to delve deeper into inflammation pathways, paving the way for breakthroughs in therapeutic development.

In addition to analyzing conventional pro-inflammatory cytokines using methods like LUMINEX or the Ella Automated Immunoassay System, our inflammation assay goes further by examining the cellular mechanisms behind inflammation through multi-parameter analyses, including gene expression studies and immunofluorescence.

  • Inflammatory panels (IFN-γ, TNF-α, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, CRP and more).
  • Cytokine and Chemokine panels (MCP-1, MCP-4, Eotaxin, IP-10, GM-CSF, IL-15, IL-16, IL-17A, TSLP, TNF- β and more)
inflammation assay

The assay can be customized to suit specific experimental requirements, allowing for the assessment of various cell types, treatments, and conditions.

Highly sensitive ELISA or Multiplex dosage of secreted cytokines by ELLA-multiplex

With multiplex capabilities, our assay enables simultaneous quantification of multiple cytokines, providing a comprehensive profile of the inflammatory response.

Exemple of Study design

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Cells amplification
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Cells seeding in 24-well plates
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Inflammation induction +/- test items
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Media collection and cells fixation
IF staining and dosages

Exemple of Assay readouts

Primary RPTEC cells response to 5 days in high glucose medium:

Cro| Inflammation assay